Monday, March 11, 2019
Practical Report
My possible results were now Bacillus cereus, Mycobacterium segments, or salaciously acidophilus. side by side(p) I preformed the acid fast staining. When preparing this slide and viewing it on fossil oil immersion, I was a little more confident nigh the forbid result yielded. You are less correspondingly to confuse a electronegative blue with a positive fuchsia or pink color, which was my issue in my gram staining process. Was teetering on whether my gram stain was purple, red, or both. My results in the acid fast staining were clearly blue, making the results negative.Looking back at the key, it narrows my possible results by one, leaving me with Mycobacterium segments, or salaciously acidophilus. My pop off staining slide was the spore stain. Here yielded a negative result. The color on my slide was red. From lab red is highlighting the cell, here green would make for the spore. Did have maybe one or two green spots, plainly chalked that up to over dripping of malachite g reen, over microwaving, or not copious water on paper towel, as my slide was cracked in half after being microwaves. Looking back again at the lab key was narrowed my choices again, ending up with salaciously acidophilus.In the end, triplet staining tests results were as follows Gram yielded positive, acid sat yielded negative, and spore standardized negative. The morphology that noticed was rod shape. I had a hard time distinguishing eagle-eyed rods from short rods. In my opinion the rods seemed short, if possible when they appeared longer they looked more like two short rods were just attached at the ends. So my position as to what my unknow bacteria is, would be Lasciviously acidophilus. My rationale for my results retort is mainly based on what was yielded from the staining Of each slide.The colored results matched to either positive or negative and the morphology just takes you a teepee closer in matching your result. The bacteria that believe my results yielded is La sciviously acidophilus. It is known as a friendly bacteria and is a very common isotropy that is found naturally in our bodies, mainly in the mouth, intestines, and the vagina. Structure judicious Lasciviously acidophilus is a single-celled prokaryotic microorganism that lacks a limpid nucleus. It also contains a cell wall, a cytoplasm membrane, a nucleoli, cytoplasm, ribosome, pill, and flagella.It is rod shaped and measures about 0. 5 to 0. 8 micrometer across by 2 to 9 mm in length. It occurs in chains and is non spore forming (Sanders 322). Lasciviously acidophilus has optimal growth at 37-42 degrees Celsius. They are sufficient to live in highly acidic environments. Prefers to grow at low pH, anything below 5. It is a motile bacteria that grows in or without the presence of oxygen. Bacteria is able to digest lactose. Culture preparations consist of dried or liquid cultures of subsisting bacteria (it can be grown on agar slants do of 2% agar in a solution of 3. 5% Lascivio usly broth).High colony counts on mediums that contain potassium acetate, dextrose, and CHOPPY to name a ewe. This bacteria is used as a proportion to prevent/ work on vaginal candidates, yeast infections of the mouth, and diarrhea. Overuse or a side personal effects that are currently known about this bacteria is constipation and flatulence. With Lasciviously acidophilus there wasnt much about the production of toxins it could cause, but more about the dittoing abilities. It aids in producing enzymes which promote digestibility, decreases the levels of toxic amines in the blood, the positive appoint goes on.When researching of other bacteria that was closely related to Lasciviously acidophilus. I was unsuccessful. just because it does grow naturally within us this bacteria works in conjunction with other bacteria and organisms within our bodies. After coming to the completion of my results until now I think was very oblivious to the bacteria that I was looking up. The more in-de pth search I went into I realized I used this bacteria, this proportion, a great deal in my profession. The physicians at my nursing home often prescribe Lasciviously acidophilus to residents that are being treated with an antibiotic.
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